NADH測定用酵素電極とその高感度化

抄録

Two kinds of immobilized enzyme layers, one containing NADH oxidase (layer I ) and the other, NADH oxidase and alcohol dehydrogenase (layer II), are prepared and are separately combined with Clark oxygen electrodes. The resulting enzyme electrodes are used for the determination of NADH. The detection limits are 2 μmol.dm^-3 for the electrode with the layer I and 20 nmol.dm^-3 for that with the layer II in the presence of ethanol. The amplification of electrode response obtained through the cyclic enzymatic reactions between NADH and NAD⁺ brings about the high sensitivity in the latter system.