ウイルス誘発腫瘍の骨髄内移植と凍結保存

抄録

Recently the solid tumor induced by Schmidt-Ruppin strain of Rous sarcoma virus was converted into the ascites form. These ascites cell tumors, approximatelly 1×10⁵ tumor cells, were inoculated into the bone marrow of CDF₁ mice through a little hole drilled below the left femur condyle. The lesion of the left femur were observed roentogenographically and histologically. After 6 or 8 weeks with the inoculation into the bone marrow, the bone destraction was seen only a little. Histologically no tumorous osteoid was found.
These ascites cell tumors survive well by dipping into the tissue culture medium and by preserving in the low temperature.