We have already shown that TCA promotes calcification of the thickened growth plate in the healing stage of HEBP-induced rachitic rats respectively. In this experiment female weanling Fisher-344 rats of 4 weeks of age were used and the experiment period was settled to be 2 weeks. Sixty mg/Kg body weight of HEBP was dosed subcutaneously, daily for the former 7 days and then 200, 400 or 600mg/kg body weight of TCA was given to the each rat of TCA group for the latter 7 days.
In the result HEBP+TCA group showed prominent mineralization and calcification of newly formed bone and caltilage of the callus in comparison to rats treated with HEBP alone at 14 days after fracture.
