ラット肝に於けるアゾ色素代謝に及ぼす自律神経系の影響

抄録

(1) Protein bound dye levels were determined in the liver of the control, the vagotomized and the splanchnictomized rats after administration of 50 mg. of 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) using a stomach tube.
The maximum binding was observed about 36 hours after administration of the dye in each group.
The maximum bound dye level in the vagotomized group was 40 per cent lower than that of the control rats, while no significant difference was found in the splanchnictomized rats.
(2) Vagotomy lowered the bound dye level in rats fed 0.05 per cent of 3'-Me-DAB for 2-3 weeks.
(3) N-Demethylase activity in the liver of vagotomized rats was 35 per cent less than that of the control rats. No significant difference was found between the control and the splanchnictomized rats.
(4) Intraperitoneal administration of adrenaline or noradrenaline resulted in the decrease of N-Demethylase activity to the extent of 35 per cent, while acetylcholine showed little effect on the activity of the enzyme.
(5) Low activity of N-Demethylase was found in the liver of adrenalectomized rats.
The depression of N-Demethylase due to adrenalectomy was prevented by adminietration of cortisone acetate.
(6) From these findings, it was concluded that the splanchnico-adrenal system possessed an inhibitory effect upon the N-Demethylase activity while the pituitary-adrenocortical system seemed necessary to maintain the normal level of the activity.