1985 Volume 25 Issue 11 Pages 881-885
The peroxidase-antiperoxidase (PAP) method was employed to determine the ultrastructural localization of Na+, K+-adenosinetriphosphatase (ATPase) in normal adult canine choroidal epithelium.
The α- and β-subunits of Na+, K+-ATPase were prepared from Na+, K+-ATPase holoenzyme, which was purified from canine renal outer medulla. Rabbit antisera to both subunits were then produced. The intense immunoreactive electron-dense products were confined along the apical plasmalemma, including that of microvilli of choroidal epithelial cells. This finding is compatible with that of the authors' previous report by light microscopic immunohistochemistry.
It can be inferred that the apical border of the epithelium is a very important site for cerebrospinal fluid secretion.
