Abstract
Volatile basic and acidic fractions obtained from Katsuobushi by steam distillation were separated by one-dimdnsional paper chromatography employing the descending technique.
In this experiment, four basic substances and five volatile fatty acids were identified by comparing the chromatogram of the sample with that of the mixture of pure substances. The basic substances thus identified are ammonia, trimethylamine, pyridine and piperidine and the volatile fatty acids are formic, acetic, propionic, butyric and lauric acids.
