Nippon Nōgeikagaku Kaishi
Online ISSN : 1883-6844
Print ISSN : 0002-1407
ISSN-L : 0002-1407
The Reversion of Starch-Iodine Color Reaction caused by an Enzyme
Part 7. Saturated Fatty Acids as the Amylose-Precipitant
Ken'ichi TAKAOKAZirô NIKUNI
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1952 Volume 26 Issue 4 Pages 186-190

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Abstract
The effects of various saturated fatty acids as amylose-precipitants were, examined. Nine even-nnm-bered saturated acids from acetic to stearic acid were chosen, and each acid was added to boiling starch paste and cooled over-night. These pastes were hydrolyzed by α-amylase during about 2 hours at room temperature. The amylose-fatty acid complex was left intact because they formed helical micell crystals, while amorphous amylopectin fraction was hydrolyzed completely. The remaining amylose-fatty acid complex was collected, washed, dried and weighed (Table 4.).
From these results, it was found that caprylic and capric acids were excellent amylose-precipitants, because they were slightly soluble in water to reach amylose molecules in the paste, and were sufficiently hydrophobic to precipitate amylose molecules. Moreover, these acids could be removed easily from the amylose complex, while the higher fatty acids were removed with great difficulties.
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© JAPAN SOCIETY FOR BIOSCIENCE,BIOTECHNOLOGY, ANDAGROCHEMISTRY
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