Abstract
The crystalline ferritin was isolated by means of fractionated precipitation of (NH4)2SO4 after water extracting the horse-spleen-protein with warmed at 50° for 10 mins., and its yield was about 0.15% of fresh matter. The isoelectric point of ferritin was about pH 4.4, and the maximum absorption band of it was at 280 mμ. The content of iron in ferritin was 20.5% per protein, and it was realized that the iron content of it was variable by the state of sample and its treatment, and that the ferritin was homogeneous electrophoretically, but in acid side under the pH 3.6 its descending patterns were heterogeneous. The spreadings which produced in this experiment were also discussed.
