1953 年 27 巻 9 号 p. 617-620
(1) In order to produce the ergot alkaloids by surface replacement cultures, it is reasonable to use such a culture medium which has rather high C/N ratio due to rather, low, nitrogen concentration.
(2) In order to produce the ergot alkaloids by surface replacement cultures, it is rea-sonable to carry out the chltivation at relatively low temperature. In general about 24° is, preferable.
(3) The alkaloid production in surface replacement ent cultures is not so much affected by-the initial pH of the culture media as in ordinary surface cultures. The alkaloid production: in the former is not, affected appreciably at the pH values between 5.0 and 6.0.
(4) The alkaloid production in surface replacement cultures is also enhanced by various, growth inhibitory substances in the culture media. But in replacement cultures, unlike in, ordinary surface cultures, the said effect can be seen for the first time when the concentr-ation of the substances is fairly increased.
(5) The mycelium of ergot fungus grown on the surface of a suitable, liquid medium, produces the alkaloids rather excellently even in submerged cultures.