Study on the Quantitative Method of Coal-Tar Dyes in Candies by the Use of Baker's Yeast as Adsorbent

Abstract

A method of quantitative determination of coal-tar dyes in candies was studied employing baker's yeast as adsorbent.
The presence of glucose (6%) markedly enhanced adsorption of dyes into cell of viable yeast, and the adsorbed dyes could be extracted with 0.5 N-NH₄OH. After neutralized and concentrated, resulting colored solution was developed on a thin-layer chromatogram, and then each band of dyes was scraped and extracted with water for colorimetry.