An Isolation Method of Protein from Single Cell by Impact-Cell-Mill

Part I. Efficiency of an Impact-Cell-Mill

Abstract

An impact-cell-mill was devised for the destruction of single cells such yeast and chlorella.
Resin bifluoride gave good results as the materials for sealing the machine and 4 or more sheets of seal packing were necessary for the durability of the machine. Sintered head alloy was suitable as the materials of a nozzle, disks and valves.
In respect to the angle of the nozzle, 15° was more suitable than 55°. The nozzle with a polished surface gave a good result. Baker's yeast could be crushed 33-44% passing once through the machine under the condition of 600-800kg/cmcm2 as sprouting pressure. The crushing energy at that time was 24-26cal/g.