An impact-cell-mill was devised for the destruction of single cells such yeast and chlorella.
Resin bifluoride gave good results as the materials for sealing the machine and 4 or more sheets of seal packing were necessary for the durability of the machine. Sintered head alloy was suitable as the materials of a nozzle, disks and valves.
In respect to the angle of the nozzle, 15° was more suitable than 55°. The nozzle with a polished surface gave a good result. Baker's yeast could be crushed 33-44% passing once through the machine under the condition of 600-800kg/cmcm2 as sprouting pressure. The crushing energy at that time was 24-26cal/g.

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An experiment to isolate protein from single cell was carried to obtain the following results. Under the condition of spouting pressure 600kg/cm², clearance between nozzle and disk 30mm, petroleum yeast and Chlorella algae could be crashed about 98%, Baker's Yeast could be done about 93%.
The most suitable clearance betwen nozzle and disk to crash single cell was 20 mm at spouting pressure 400kg/cmcm2, and 30mm at over 500 kg/cm².
When the concentration of the suspension of single cells were high, it's viscosity showed above 150cp, and Impact-Cell-Mill could not show high pressure to envelopm any small air bladder cells resulting in the reduction of the destruction ratio of single cell.

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It is known that nitrate in tomato fruit causes heavy tin-dissolving in canned tomato juice.
In the previous paper, it was reported that the application of the top-dressing of nitrogen increased nitrate content in tomato fruit. The present study was carried out to investigate the influence of the application time of the top-dressing of nitrogen on the accumulation of nitrate in tomato fruit.
While nitrate in soil increased by the application of the top-dressing, the content varied remarkably with the time of application. Considerable amount of nitrate was detected in the fruit grown on high level of nitrate in the soil. Nitrate contents in both soil and fruit increased when the top-dressing had been applied shortly before the beginning of harvest, or three times during the growing period.

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In order to find the factors concerning the accumulation of nitrate in tomato fruit, the influences of the inorganic (ammonium sulfate) and the organic (stable manure) nitrogen fertilizers were studied by using flooded and unflooded fields. Flooding during the winter season was purported to control pathogenic fungi.
The nitrate content in the soil supplied with the inorganic nitrogen was higher than that supplied with the organic nitrogen, but appreciable amount of nitrate was detected even in the latter. In the flooded field, the nitrate content in the soil and the fruit which had been supplied with the inorganic nitrogen were higher than that supplied with the organic nitrogen. In the unflooded field, however, the nitrate content in the fruit supplied with the inorganic nitrogen was lower than that supplied with the organic nitrogen, in spite that the content in the soil supplied with the inorganic was higher than that supplied with the organic nitrogen.
The nitrate contents in the soil and tomato fruit in the flooded field were lower than that in the unflooded field, especially when the organic nitrogen had been supplied.

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Enzyme activity of Haku-Shoyu-Koji increased rapidly from twenty to forty hours after inoculation. Phosphatase showed a slight increase after forty hours, whereas amylase, acidic and alkaline protease reached to the maximum after forty-eight hours. Neutral protease showed maximum after sixty hours.
From the results obtained, it seemed that the forty-eight hours was the most desirable cultivation time for making Haku-Shoyu-Koji.

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The low temperature injuries were studied in white peach "Okubo" fruits, in relation to temperatures and the delayed storage.
Fruits ripened in 7-9 days at 20°C, 17-21 days at 10°C and the ripening was suppressed at 2.8°C. More injuries developed at 2.8°C than at 1°, and 10°C, and the development of injuries was reduced by the delayed storage.
Fruits seemed to be more susceptible to low temperature injuries at 3-5°C than at 0-1°C. The best storage conditions seemed to be in the delayed storage at 0-1°C.

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