High Performance Liquid Chromatographic Determination of Saccharides in Dairy Products

Abstract

Using a LiChrosorb NH₂(10μm)column, fructose, glucose, lactose and sucrose were separated and determined quantitatively. Samples were dissolved in water or diluted with water if necessary, treated with 80% ethanol, centrifuged at 1150g, and then the supernatants were injected into a liquid chromatograph without further purifications. Analytical time was less than 10 minutes. The average recovery of lactose added to samples was 102.3%. Precision study showed when the sugar content was more than 1%, the average of coe fficient of variations was 4.5%.