Microscopic structures of soybean cotyledonary tissues were investigated, using both Chinese soybean stored at 35°C, 80% RH for 10 months(Stored beans)and that stored in a refrigerator(Control beans) as materials. In stored beans, losses and breakage of cell structure, deformation of protein bodies and disappearance of starch granules were observed, moreover, the change of the staining behavior of spherosomes with osmium was found clearly. Through the estimation of reducing power by O
2-Shiff reaction and by the formation of ferricyan blue, the difference between stored and control beans was recognized definitely. In this experiment, slices of Epon block initially prepared for a transmission electron microscope were stained with Sudan black 10B, Comassie Brilliant Blue and periodic acid-Schiff reaction, and successfully observed under a light microscope.
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