1981 Volume 28 Issue 3 Pages 142-146
A method to prepare immobilized urease on the inner wall of a glass tube by radiation-polymerization under frozen state was investigated. As a part of a continuous flow analyzer. i.e. Technicon Auto Analyzer II, the immobilized urease tube of 1cm length was set and used for the routine determination of urea. This flow-through system could measure urea concentration of up to 30mM at rate of 30 samples per hour. The system were possible to assay 2000 to 3000 samples, continuously in practice. The activity of the urease tube stored in a refrigirator maintained 94% of the initial activity after 115 days.
