Determination of Urea Using Immobilized Urease and Ammonia Electrode
Immobilization of Enzyme by Radiation-Polymerization and Its Application to Analysis Part V
1981 Volume 28 Issue 3 Pages 147-151
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1981 Volume 28 Issue 3 Pages 147-151
A simple method to determine urea using an ammonia electrode combined with the tubular immobilized urease prepared by radiation-polymerization under frozen state was investigated. There was not much difference among three commertial electrodes, in their response or diffusion properties to ammonia. In flow-through system of the ammonia electrode, 2 to 4 minutes was needed for each sample to measure ammonia. Moreover, the time to wash out adsorbed ammonia out of the immobilized ureaSe tube was also required 2 to 4 minutes. According to those results, a simple device of urea determination which required 10 minutes for one procedure was constructed. This device provided a linear calibration line in the range of 5×10-5 to 10-3M urea, and the slope was caliculated to be 24mV/log [urea].
