Abstract
Quantitative determination of β-phenethyl isothiocyanate was investigated employing GC equipped with FID and FPD. Column bath temperatures were isothermal at 120°C and programmed from 80 to 160°C. When n-hexane was used as a solvent and thianaphthene was used as an internal standard, relation curves between peak area of β-phenethyl isothiocyanate/that of thianaphthene (KI, AI/Astd) and concentration of β-phenethyl isothiocyanate described straight line using FID. Using FPD, however, these curves described parabola, and relation curves between √KI and concentration of β-phenethyl isothiocyanate described straight lines. Accordingly, it was concluded that possibility of quantitative determination of β-phenethyl isothiocyanate was proved using both FID and FPD. Contents of β-phenethyl isothiocyanate in 5 kinds of horseradish flour hydrolysates were 0.128-0.241 wt% in the case of FPD (programmed).
