Abstract
1. Three groups of indivi d u als of Oryzias latipes were kept for five hours in the water of the temperature 0°C.,31°-32°C. and 18°-20°C. respectively. The activity of alkaline and acid phosphatase in liver cells of these fish was observed.
2. Alkaline phosphatase:
In the low-temperature experiment, the activity is intensified in nucleolus and bile capillary, and is reduced in cytoplasm.
In the high-temperature experiment, the a c tivity is remarkably intensified in nucleus, in blood vessel wall, in portion of cytoplasm in contact with blbod vessel, and in bile capillary. Cytoplasm of hepatic cells contains vacuoles, in which no positive reaction is obse rvable, but around which strong activity is shown.
3. Acid phosphatase:
In the low-temperature experiment, the activity of the, nucleus is increased to some extent, while the reaction in cytoplasm and bil e capillary decreases.
In the high-temperature experiment the activity in nucleus is slightly intensified, but the activity in cytoplasm is diminished. Within cytoplasm, vacuoles exist, which show no positive reaction.
