Abstract
Saccharomyces cerevisiae can synthesize thiamine from its pyrimidine and thiazole moieties but the rate of synthesis varied with cells, harvested in different media and reaction media for synthesis. The highest yield on synthesis was demonstrated by cells harvested in Atkin's medium and by the biosynthesis in Reader's medium. There was found higher yield on synthesis by cells harvested at the exponential phase than those at the stationary phase. The thiamine synthesized, was always found in cells, not in medium and about 1/4 of the thiamine was shown as its phosphorylated forms.
