Postembedding Staining of Several Rat Cells with Lectin Ferritin Conjugates

Abstract

Postembedding staining of intracellular carbohydrates of various rat cells embedded in Epon was carried out with three kinds of lectin-ferritin conjugates. These conjugates stained various intracellular carbohydrate containing components. Free ferritin showed almost no binding to these structures and staining with the conjugates was inhibited by the addition of appropriate competitive sugars to the staining solutions. This method is very simple and can be easily reproduced.