Immunoperoxidase Techniques with a Combined Use of PAP and ABC Procedures: Application to lmmunohistochemical Studies Using Monoclonal Antibodies

Abstract

A sensitive immunoperoxidase technique was developed by combining PAP and ABC procedures for the purpose of applying them to immunohistochemical study using monoclonal antibodies. Using the monoclonal antibody to prostatic acid phosphatase as the primary layer, the sites of acid phosphatase were visualized in nontumorous prostate tissue even at a 1: 12,500 dilution of the antibody, followed by application of the PAP + ABC procedure. This technique was found to be more sensitive than either PAP or ABC procedure alone. The high sensitivity obtained with this procedure may be attributable to multiple HRP molecules bound to the antibodies through the treatment with both PAP and ABC complexes.