Abstract
Summary: As a preliminary study of the double labeling technique, single administrations of fluorescent dye, Fast Blue or Nuclear Yellow (Hoechst S 769121) were given to the left extraocular muscles of the rat respectively. The distribution patterns were then investigated. The author found that labeled neurons were in the bilateral oculomotor nucleus, trochlear nucleus, ipsilateral abducens nucleus and accessory abducens nucleus. In the ipsilateral culomotor nucleus, labelled neurons grouped as the rostro-caudally arranged subnuclei: the inferior rectus was found in the ventral portion, the medial rectus situated mostly inner part, the inferior oblique located dorsally, the superior rectus is distributed center area.
Compared to the author's previous findings using HRP, the single administration of Fast Blue yielded similar results but the single administration of Nuclear Yellow revealed a rather more limited distribution.
