Abstract
An improved method for cutting thick serial sections for light microscopy from tissue embedded in Epon is described. Tissue blocks were embedded in an Epon mixture that was suitable for both thick and ultrathin serial sections as the result of a number of tests. Thick serial sectioning should be done on a small sliding (Schanze-type) light microtome with the razor blade for light microtomes. The sections produced were picked up serially on a glass slide and observed under a light microscope. When necessary, sections were re-embedded in Epon, and ultrathin sections were cut on an ultramicrotome and observed under an electron microscope.
