Okajimas Folia Anatomica Japonica
Online ISSN : 1881-1736
Print ISSN : 0030-154X
ISSN-L : 0030-154X
Volume 65, Issue 5
Displaying 1-7 of 7 articles from this issue
  • Masatake IMAI, Taizo SHIBATA, Keiichi MORIGUCHI
    1988 Volume 65 Issue 5 Pages 245-253
    Published: 1988
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    1. The glands, distributed in the narrow area contiguous to the proventriculus have been regarded as cardiac glands that secreted serum or mucus. However, they contain pepsinogen granules besides several kinds of mucosubstances. 2. Several simple tubular glands, distributed in the immediate vicinity of the proventriculus (A glands), have few parietal cells, and the glands strongly reacted to PAS and AB (pH 2.5), but not to AB (pH 0.5). 3. The A glands contain sialomucin in the glandular neck, weak acid and neutral mucopolysaccharides in the body and base of the glands, but no strong acid mucopolysaccharide. 4. The A glands contain fine pepsinogen granules in the glandular cells of the basal portion. 5. A great number of parietal cells appear in the glands contiguous to the A gland zone separated by less than 10mm from the proventriculus (B glands). 6. B glands contain the same mucosubstances as in the A glands but no strong acid mucopolysaccharide. 7. The B glands also contain pepsinogen granules in the basal portion of the glands. 8. The A and B glands belong to the same category, however, the A glands are slightly more undifferentiated than the B glands. Namely, a few of the parietal cells and a large number of the strongly PAS- and AB (pH 2.5)-positive cells in the A glands are a symbol of immaturity. 9. There is a mixed zone in both gland regions. 10. The fundic glands have a large number of parietal cells like the B glands. 11. The pepsinogen granules in the fundic glands surpass the B glands in number and size. 12. The fundic glands have no weak and neutral mucopolysaccharides in the glandular lower portion which are contained in the lower portion of the B glands. The lack of the mucosubstances is an indication of the maturity of the glandular cells. 13. Based on the above findings, the B and fundic glands are of the same type, though, the latter is mature and the former is in an immature stage. 14. The so-called cardiac glands are A and B glands. Accordingly, they are not special kinds of glands but undifferentiated fundic glands.
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  • In vitro Quantitative Autoradiographic Investigation
    Kiminao MIZUKAWA, Nagayasu OTSUKA, Kumiko HABA, Norio OGAWA
    1988 Volume 65 Issue 5 Pages 255-265
    Published: 1988
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    The binding of 3H-labeled L-quinuclidinylbenzilate (QNB) sites was studied in cryosections of the rat spinal cord (cervical, thoracic and lumbar cords) by in vitro macroautoradiography in an attempt to elucidate the distribution of muscarinic cholinergic (mACh)receptors. In this study, transverse sections as well as longitudinal sections of the thoracic cord were employed. A moderately high density of QNB binding sites could be seen in the gray matter of the spinal cord, especially in the marginal regions of the dorsal horn and around motor neurons in the ventral horn. In the lateral horn of the thoracic cord, a moderately high density of QNB binding sites was segmentally observed. By image analysis with a RAS 1000 (Amersham), color coded autoradiographic images which enhanced the small differences in optical density could be easily obtained, and quantitative analysis of the QNB binding sites in small-sized regions of the spinal cord was also performed. The highest densities of mACh receptors in the spinal cord were localized in the substantia gelatinosa of the dorsal horn and the region of motor neurons of the ventral horn. These findings suggest that while mACh receptors are related with motor functions, they are deeply related with sensory and autonomic functions.
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  • Shigemitsu YOSHIDA, Hayato OHSHIMA, Shigeo KOBAYASHI
    1988 Volume 65 Issue 5 Pages 267-281
    Published: 1988
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Dental pulp, Microvasculature, Tooth development, Dentinogenesis, Vascular cast Summary: The developmental changes of the vascular supply in the dental pulp were studied in molars of rats from the 19-day embryo to 21 days after birth employing the vascular casting/scanning electron microscope method. In the 19-day embryo, terminal pulpal capillaries were observed in the inner region of the dental papilla and formed a coarse, flattened vascular network consisting of thick capillaries. With the beginning of dentinogenesis, the terminal pulpal capillaries invaded into the odontoblastic layer forming hairpin-like loops and narrowing their lumina. At 21 days after birth, they formed a dense, flattened vascular network consisting of thin capillaries between the odontoblasts, only about 5μm from the predentin. These findings indicate that the terminal pulpal capillaries play an active role in dentin formation.
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  • Fumihiko SASAKI
    1988 Volume 65 Issue 5 Pages 283-289
    Published: 1988
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    An improved method for cutting thick serial sections for light microscopy from tissue embedded in Epon is described. Tissue blocks were embedded in an Epon mixture that was suitable for both thick and ultrathin serial sections as the result of a number of tests. Thick serial sectioning should be done on a small sliding (Schanze-type) light microtome with the razor blade for light microtomes. The sections produced were picked up serially on a glass slide and observed under a light microscope. When necessary, sections were re-embedded in Epon, and ultrathin sections were cut on an ultramicrotome and observed under an electron microscope.
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  • Seiichiro KITAMURA, Takahiko NISHIGUCHI, Kimio OGATA, Akira SAKAI
    1988 Volume 65 Issue 5 Pages 291-301
    Published: 1988
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    The distribution of the neurons of origin of the internal ramus of the rabbit accessory nerve in the nudeus ambiguus was studied with injection of HRP into both the inferior vagal ganglion and the muscles of the palate and pharynx following the intracranial severings of the ipsilateral rootlets of the vagus and glossopharyngeal nerves and/or those of the cranial root of the accessory nerve. The neurons of origin were found to be located in the subnucleus DiG at the level of the caudal half of the nucleus ambiguus, with their number being about one-tenth of the total number of the constituent neurons of this nucleus. DiG is the abbreviation for a cell grouping formed by a diffuse arrangement of the largest neurons of the nucleus ambiguus, and which occupies the caudal two-thirds of the nucleus. The neurons of origin were intermingled with the vago-glossopharyngeal rootlet neurons at the rostral levels of the DiG. At the level of its most caudal one-third, however, that subnucleus was occupied only by the neurons of origin of the internal ramus.
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  • Takashi NAKANO, Yoshie IWAMA, Kaori HASEGAWA
    1988 Volume 65 Issue 5 Pages 303-313
    Published: 1988
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Keratinization, Keratohyalin granule, Epithelium, Pharynx, Mouse Summary: In the mouse laryngopharynx, the stratified squamous epithelium was studied by transmission electron microscope in order to determine its morphological characteristics. The superficial cells were filled with opaque filaments embedded in a less opaque interfilamentous substance. Further, they had thickened plasma membrane and contained a fairly large number of keratohyalin granules showing ultimate stages transforming into interfilamentous substance. It is suggested that the superficial cells appear to represent the transitional stage in the keratinizing process, and had morphologically close resembrance to both the granular cells and the cornified ones in keratinized epithelia.
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  • Toshio ITO
    1988 Volume 65 Issue 5 Pages 315-335
    Published: 1988
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    In human axillary, perianal and nasal Ala skins eccrine (e) and apocrine (a) sweat glands were light microscopically studied and five cases of the so-called "mixed sweat gland" (mixed sweat glands I, II, III, IV and V) were observed. In the mixed sweat glands the secretory cells of the aand e-sweat glands co-existed in one and the same gland in a variety of fashion either being restricted in a small portion of the secretory tubules or spreading in the wide range of the gland in contradiction to the concept of Schiefferdecker that both sweat glands represent perfectly disconnected, quite distinct glands. Our light microscopic studies have revealed that the superficial cells of the e-sweat gland exhibited also the picture of the apocrine secretion, though the activity of secretion was inferior to that of the a-sweat gland cells. The presence of the mixed sweat glands naturally suggest that between the both types of the sweat gland the transformation might take place. The mixed gland IV showed especially the sequence of the gradual transformation of the a-sweat gland into the e-sweat gland. The mixed sweat gland II and V suggested, in agreement with the mixed gland IV, also the transformation of the a-sweat gland into the e-sweat gland, since in the secretory epithelium of a-gland appeared the clear basal cells holding secretory canaliculi between themselves. The clear basal cells might derived from myoepithelial cells (Yasuda,1959). In the mixed sweat gland II the clear basal cells appeared close to the membrana propria as if to occupy the position of the myoepithelial cells. In the mixed sweat gland I, II, IV and V it was thought necessary that luminal a-gland cells turned into the superficial cells of the e-gland for complete transformation of the a-sweat gland into the e-sweat gland; the Yasuda's proposal (1959) that the possibility of transition of a-sweat gland cells into the dark superficial cells of the e-sweat gland was rather easy to understand.
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