Ear Research Japan
Online ISSN : 1884-5797
Print ISSN : 0288-9781
ISSN-L : 0288-9781
セロイジン切片法によるネコ蝸牛の走査電顕的観察
森田 浩史水田 邦博星野 知之
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ジャーナル フリー

1990 年 21 巻 1 号 p. 101-102

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The organ of Corti was studied in cats using celloidin thick serial sectioning method. The fixed temporal bones were decalcified and embedded in celloidin, then thick serial sections (100-150 μm) were cut parallel to the basilar membrane. After removing celloidin, the sections were freeze-dried in t-butyl alcohol, coated with gold and observed under a scanning electron microscope. The base and the ceiling of the Corti's tunnel and the Nuel's space, the nerve fibers and the nerve endings were constantly and easily observed. Though some outer hair cells had been deformed, the nerve fibers were well preserved for analysis.
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© 日本耳科学会
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