At J-PARC, beam commissioning of Materials and Life Science Experimental Facility (MLF) has been started in May 2008. With only one shot of the proton beam, we succeeded to deliver the proton beam to the neutron production target because of the good preparation of the monitor and the magnet. We also succeeded, by the only one shot of the beam, to recognize that the observed performance of the neutron beam agreed well with the design. Furthermore, we successfully produced the muon beam, so that the fundamental mission of MLF as a secondary beam supplier was completed. In December 2008, MLF was opened for users to use the neutron and the muon beam.