Plankton and Benthos Research
Online ISSN : 1882-627X
Print ISSN : 1880-8247
ISSN-L : 1880-8247
Note
Molecular-based method to distinguish sympatric deep-sea whelks, Buccinum striatissimum and Buccinum tenuissimum in the Sea of Japan
Akira IguchiMasahiro UenoTsuneo MaedaTakashi MinamiHaruhiko ToyoharaIsao Hayashi
著者情報
ジャーナル フリー

2008 年 3 巻 2 号 p. 101-103

詳細
抄録
Buccinum striatissimum and Buccinum tenuissimum are commercially important whelks in the Sea of Japan. B. striatissimum is very similar to B. tenuissimum and their morphological variations make the identification of these species difficult, especially in case that these species are caught together because they are subject to the same environmental factors. In order to develop an easy and objective method to delineate these species, we utilized the difference of mitochondrial 16SrDNA of these two species. As a result, we could easily distinguish these two species by PCR-RFLP method using the difference of this region and AseI restriction endonuclease. In addition, we succeeded in distinguishing sympatric samples of these species, which suggests that these two species are likely to be reproductively isolated although the possibility of hybridization can not be completely ruled out.
著者関連情報
© 2008 The Plankton Society of Japan, The Japanese Association of Benthology
前の記事
feedback
Top