内視鏡生検による十二指腸HCO₃^-測定系の確立について

抄録

This study was designed to characterize the mechanisms available for bicarbonate transport across the apical membrane using duodenal brush border membrane vesicles (BBMVs) , including the morphological findings. BBMVs were purified using a double Mg²⁺ precipitation technique.
Electron microscopy of the final pellet showed 11.7±2.9 fold in Al-p and 12.6±1.8 fold in LAP. The time course of ³⁶Cl uptake into BBMVs demonstrated a good response curve and equilibrated to the intravesicular space over 120 min.
In this present study, these BBMVs were purified and showed a stabilized structure. Therefore, these results suggest that this model was useful for studying HCO₃^- secretion and ulcer etiology.