Cell Adhesion on Polyimide Surface Patterned by Ion-irradiation

Abstract

In this study, we prepared a novel fluorinated polyimide surface using both a rubbing machine with a rubbing cloth and an ion-beam irradiation apparatus for cell culture. We determined the surface properties of the rubbed film using an atomic force microscope and contact angle measurements. In addition, we evaluated the cell adhesion behavior on the polyimide film using a phase contrast microscope. Interestingly, a polyimide surface having a micrometer-scale grooved pattern was prepared by the rubbing method, and the morphologies of cells attached to the rubbed surface were three-dimensional multicellular spheroids. In addition, the cells were selectively adhered on the ion-irradiated surface, suggesting that the polyimide surface may become a novel cell culture method.