Journal of Photopolymer Science and Technology
Online ISSN : 1349-6336
Print ISSN : 0914-9244
Rapid Photopolymerization of Oligodeoxynucleotides by 3-Cyanovinylcarbazole mediated DNA Photocrosslinking
Shigetaka NakamuraKenzo Fujimoto
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2014 Volume 27 Issue 4 Pages 485-490

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Abstract

We proposed rapid photopolymerization of oligodeoxynucleotides (ODNs) by 3-cyanovinylcarbazole (CNVK), which photoirradiates the crosslinking between DNA strands at 366 nm. This photopolymerization of ODNs using CNVK was completed by the photoirradiation for within two seconds, the reaction rate of which is faster than any other method such as enzymatic and chemical ligation. Moreover, the photopolymerized DNA structure was degraded to the monomer ODN by photoirradiation at 312 nm. The photopolymerized DNA structure has B-form the same as the native helical duplex structure and its structure attained thermal resistance when maintained at a temperature of 60°C. Sequence specific photopolymerization incorporating micro RNA was also successful.

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© 2014 The Society of Photopolymer Science and Technology (SPST)
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