Abstract
Dimyristoylphosphatidylcholine (DMPC) and 1, 2-dimyristoylamido-1, 2-deoxyphosphatidylcholine (D14DPC) were studied with 1H- and 31P- NMR. By using the nuclear Overhauser enhancement difference spectra (NOEDF) and the nuclear Overhauser effect spectroscopy (NOESY) in 1H NMR, we found that the 1H NMR chemical shift of N+(CH3)3 of DMPC liposome is different from that of the D14DPC liposome. It was also found that in D14DPC/DMPC mixed liposome D14DPC might prefer to locate in outer leaflet of the lipid bilayer similarly to the case of sphingomyelin (SPH). 1H-Detected 13C NMR has been successfully used to measure the 13C spin-lattice relaxation time (T1) of N+(CH3)3 of DMPC liposome. The sensitivity with this method was higher by 16 times compared with that of ordinary inversion recovery (IR) method.
