Dimyristoylphosphatidylcholine (DMPC) and 1, 2-dimyristoylamido-1, 2-deoxyphosphatidylcholine (D14DPC) were studied with 1H- and 31P- NMR. By using the nuclear Overhauser enhancement difference spectra (NOEDF) and the nuclear Overhauser effect spectroscopy (NOESY) in 1H NMR, we found that the 1H NMR chemical shift of N+(CH3)3 of DMPC liposome is different from that of the D14DPC liposome. It was also found that in D14DPC/DMPC mixed liposome D14DPC might prefer to locate in outer leaflet of the lipid bilayer similarly to the case of sphingomyelin (SPH). 1H-Detected 13C NMR has been successfully used to measure the 13C spin-lattice relaxation time (T1) of N+(CH3)3 of DMPC liposome. The sensitivity with this method was higher by 16 times compared with that of ordinary inversion recovery (IR) method.
This paper describes the aseismic performance of the Concrete Mat Works with Wedge Block Joints System (WBJS Works) on the basement of a concrete gravity dam. The WBJS Works were developed by the author on collaboration with other researchers to treat the basements of dams that have large-scale faults or fracture zones. These Works were first used in the Dondo Dam located in southern Hyogo Prefecture and were subjected to the trial of the Hyogo-ken Nanbu Earthquake that took place on January 17, 1995. The excellent aseismic characteristics of the WBJS Works were proved through the response behavior of this dam during the Earthquake.
Testicular and haemolymph ecdysteroid levels in the silkmoth, Bombyx mori, were measured from the beginning of the last larval instar until 1 day before adult eclosion by Radioimmunoassay. The amount of ecdysteroids in testes just after the 4th ecdysis was about 150 pg/pair and rapidly decreased by Day 2. Afterwards, ecdysteroids titre increased gradually. An abrupt increase of ecdysteroids titre was observed during the wandering stage. After pupation, the level of ecdysteroids became increasingly detectable on Day 3 of the pupal stage and it decreased rapidly thereafter. The main component of ecdysteroids in larval testes was 20-hydroxyecdysone (about 2/3 of the total) and ecdysone. An unknown ecdysteroid which eluted at the same retention time as authentic 20-hydroxyecdysone-22-acetate was also detected. The fluctuation pattern of ecdysteroids in the haemolymph closely paralleled that of the testes, although the titres were much higher than those of testes.