Dimyristoylphosphatidylcholine (DMPC) and 1, 2-dimyristoylamido-1, 2-deoxyphosphatidylcholine (D
14DPC) were studied with
1H- and
31P- NMR. By using the nuclear Overhauser enhancement difference spectra (NOEDF) and the nuclear Overhauser effect spectroscopy (NOESY) in
1H NMR, we found that the
1H NMR chemical shift of N
+(CH
3)
3 of DMPC liposome is different from that of the D
14DPC liposome. It was also found that in D
14DPC/DMPC mixed liposome D
14DPC might prefer to locate in outer leaflet of the lipid bilayer similarly to the case of sphingomyelin (SPH).
1H-Detected
13C NMR has been successfully used to measure the
13C spin-lattice relaxation time (
T1) of N
+(CH
3)
3 of DMPC liposome. The sensitivity with this method was higher by 16 times compared with that of ordinary inversion recovery (IR) method.
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