植物組織培養
Online ISSN : 1884-7706
Print ISSN : 0289-5773
ISSN-L : 0289-5773
Duboisia 属植物培養細胞のプロトプラストの培養と再分化
北村 美江森川 隆梅野 尚生三浦 博史
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1989 年 6 巻 3 号 p. 172-174

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Protoplasts isolated from suspension cells of D. myoporoides, D. leichhardtii and D. hopwoodii were divided and formed colonies in the modified B5 medium, though there were differences among species with respect to culture conditions required for colony formation. Colonies developed into calli by the two-step culture methods. The calli of D. myoporoides and D. leichhardtii, but not of D. hopwoodii, regenerated shoots which gave rise to plants.

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