Difference in the Ability of Initiation and Maintenance of Embryogenic Cultures among Sugi (Cryptomeria japonica D. Don) Seed Families

抄録

Zygotic embryos of Cryptomeria japonica were collected from 20 clones and cultured on half-concentration of Murashige and Skoog medium containing 10μM2, 4-dichlorophenoxyacetic acid to examine the differences in embryogenic tissue (ET) initiation among seed families. ETs were obtained in all the seed families tested, with an overall initiation rate of 45.6% (689 from 1512 explants). However, initiation rates and maintenance rates differed considerably among seed families. They varied from 7.5% to 78.5% and from 1.2% to 27.8%, respectively.