Genetic Transformation in Vaccaria pyramidata Using Agrobacterium rhizogenes

抄録

To establish a transformation system and contribute to breeding of dwarf cultivars in Vaccaria pyramidata using Agrobacterium rhizogenes, regeneration from a hairy root and traits of the regenerant were examined. The hairy roots were induced and actively grew on phytohormone-free 1/2MS media solidified with 2% gellan gum. Shoots regenerated from the root through callus on the 1/2MS media supplemented with 1.0-2.0mgl^-1 of zeatin and with 0.5mgl^-1 of IBA and 1.0mgl^-1 of zeatin. Plant height of a potted transformant was lower than those of non-transformants, and leaves of the transformant curled. Differences in morphologies and sizes of flowers in both types of the plants were little, although the starting date of flowering was 44 days later in the transformant than non-transformants. The results throw a new light on genetic engineering and the breeding of dwarf cultivars in Vaccaria.