Detection of male gamete-specific histones in angiosperms

Ichiro Tanaka

doi:10.5685/plmorphol.10.60

被子植物の雄性配偶子特異的ヒストンの検出

田中一朗

著者情報

ジャーナルフリー

1998 年 10 巻 1 号 p. 60-67

DOI https://doi.org/10.5685/plmorphol.10.60

詳細

発行日: 1998 年受付日: - J-STAGE公開日: 2010/06/28 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2010/06/28 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Key Words: chromatin, generative nucleus, histone variants, Lilium, pollen
Right : chromatin, generative nucleus, histone variants, Lilium, pollen

訂正日: 2010/06/28 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : E
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Right : LaFountain, K. L., Mascarenhas, J. P. (1972)Isolation of vegetative and generative nuclei from pollen tubes. Exp. Cell Res. 73: 233-236.
Mascarenhas, J. P. (1990)Gene activity during pollen development. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41: 317-338.
Meistrich, M. L., Bucci, L. R., Trostle-Weige, P. K., Brock, W. A. (1985)Histone variants in rat spermatogonia and primary spermatocytes. Dev. Biol. 112: 230-240.
McCormick, S. (1991)Molecular analysis of male gametogenesis in plants. Trends Genet. 7: 298-303.
Poccia, D. (1986)Remodeling of nucleoproteins during gametogenesis, fertilization, and early development. Int. Rev. Cytol. 105: 1-65.
Reynolds, T. L., Raghavan, V. (1982)An autoradiographic study of RNA synthesis during maturation and germination of pollen grains of Hyoscyamus niger. Protoplasma 111: 177-188.
Reynolds, W. F., Wolfe, S. L. (1984)Protamines in plant sperm. Exp. Cell Res. 152: 443-448.
Russell, S. D. (1991)Isolation and characterization of sperm cells in flowering plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42: 189-204.
Saito, C., Fujie, M., Sakai, A., Kuroiwa, H., Kuroiwa, T. (1998)Detection and quantification of rRNA by high-resolution in situ hybridization in pollen grains. J. Plant Res. 111: 45-52.
Sheridan, W. F. (1973)Nonaqueous isolation of nuclei from lily pollen and an examination of their histones. Z. Pflanzenphysiol. 68: 450-459.
Tanaka, I. (1988)Isolation of generative cells and their protoplasts from pollen of Lilium longiflorum. Protoplasma 142: 68-73.
Tanaka, I. (1993)Development of male gametes in flowering plants. J. Plant Res. 106: 55-63.
Tanaka, I. (1997)Differentiation of generative and vegetative cells in angiosperm pollen. Sex. Plant Reprod. 10: 1-7.
Tanaka, I., Kitazume, C., Ito, M. (1987)The isolation and culture of lily pollen protoplasts. Plant Sci. 50: 205-211.
Tanaka, I., Ono, K., Fukuda, T. (1998)The developmental fate of angiosperm pollen is associated with a preferential decrease in the level of histone H1 in the vegetative nucleus. Planta 206: 561-569.
Taylor, L. P., Hepler, P. K. (1997)Pollen germination and tube growth. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 461-491.
Twell, D., Park, S. K., Laianne, E. (1998)Asymmetric division and cell-fate determination in developing pollen. Trends Plant Sci.3:1380-1385.
Ueda, K., Tanaka, I. (1994)The basic proteins of male gametic nuclei isolated from pollen grains of Lilium longiflorum. Planta 192: 446-452.
Ueda, K., Tanaka, I. (1995a)Male gametic nucleus-specific H2B and H3 histones, designated gH2B and gH3, in Lilium longiflorum. Planta 197: 289-295.
Ueda, K., Tanaka, I. (1995b)The appearance of male gamete-specific histones gH2B and gH3 during pollen development in Lilium longiflorum. Dev. Biol. 169: 210-217.
Xu, H., Swoboda, I., Bhalla, P. L., Sijbers, A. M., Zhao, C., Ong, E-K., Hoeijmakers, J. H. J., Singh, M. B. (1998)Plant homologue of human excision repair gene ERCCI points to conservation of DNA repair mechanisms. Plant J. 13: 823-829.

抄録

要旨:我々は、テッポウユリの花粉から雄性配偶子核(雄原核)を効率的に単離する方法を開発することによって、雄原核クロマチンのヒストン組成を一般の体細胞核と詳細に比較することを可能にした。その結果、雄原核は通常の5種類の体細胞型ヒストンの他に3種類の特異的なヒストン変種(p22. 5, p21, p18. 5)を含むことが示された。そこで、これらのヒストン変種に対する特異抗体を用いることによって、それらの雄性配偶子核(雄原核・精核)特異性が間接蛍光抗体法によって実証されたとともに、それらのcDNAが単離され、それぞれの相同性からgH2B, gH3, gH2Aと命名された。これらは、高等植物で最初の雄性配偶子特異的ヒストンであり、雄性配偶子核特有のクロマチンの凝縮に関与していると推察されている。

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