Behavior of mitochondria in synchronized cells of Chlamydomonas reinhardtii

Tomoko Ehara

doi:10.5685/plmorphol.10.50

同調培養Chlamydomonasにおけるミトコンドリアの動態

江原友子

著者情報

ジャーナルフリー

1998 年 10 巻 1 号 p. 50-59

DOI https://doi.org/10.5685/plmorphol.10.50

詳細

発行日: 1998 年受付日: - J-STAGE公開日: 2010/06/28 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2010/06/28 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Key words: Chlamydomonas reinhardtii, synchronized culture, giant mitochondrion , behavior of mitochondria
Right : Chlamydomonas reinhardtii, synchronized culture, giant mitochondrion, behavior of mitochondria

訂正日: 2010/06/28 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E

Right : Arnold C. G., Schimmer O., Schö tz F.und Bathelt H. (1972)Variety of mitochondrial shapes, sizes and volumes in Chlamydomonas reinhadtii. Arch.Mikrobiol., 81: 50-67.
Bereiter-Hahn J. (1976)Dimethylaminostyrylmethylpyridiniumiodine(DASPMI)as a fluorescent probe for mitochondria in situ. Biochim. Biophy. Acta, 423: 1-14.
Blank R. and Arnold C. G. (1980b)Variety of mitochondrial shapes, sizes and volumes in Chlamydomonas reinhardtii. Protoplasma, 104: 197-191.
Brandt P. (1975)Zwei Maxima plastidarer DNA-Synthese mit unterschiedlicher Lichtabhangigkeit im Zellzyklus von Euglena gracilis. Planta, 124: 105-107.
Calvayrac R., Butow R. A. and Lefort-Tran M. (1972)Cyclic replication of DNA and changes in mitochondrial morphology during the cell cycle of Euglena gracilis(Z). Exptl. Cell Res., 71: 422-432.
Chiang K. S. and Sueoka N. (1967)Replication of chloroplast DNA in Chlamydomonas reinhardtii during vegetative cell cycle: its mode and regulation. Proc. Natl. Acad. Sci. USA, 57: 1505-1513.
Cook J. R. (1966a)The synthesis of cytoplasmic DNA in synchronized Euglena. J. Cell Biol., 29: 369-373.
Ehara T., Osafune T. and Hase E. (1985)Interactions between the nucleus and cytoplasmic organelles during the cell cycle of Euglena gracilis in synchronized cultures III. Associations between the nucleus and chloroplasts at an early stage in the cell cycle under photoorganotrophic conditions(PartII)Plant Cell Physiol., 26: 1155-1165.
Ehara T., Ogasawara Y., Osafune T. and Hase E.(1990a)Behavior of chloroplast nucleoids during the cell cycle of Chlamydomonas reinhardtii(chlorophyta)in synchronized culture. J. Phycol., 26: 317-323.
Ehara T., Osafune T. and Hase E. (1990b)Interactions between the nucleus and cytoplasmic organelles during the cell cycle of Euglena gracilis in synchronized cultures IV.An aggregate form of chloroplasts in association with the nucleus appearing prior to chlo roplast division. Exptl. Cell Res., 190: 104-112.
Ehara T., Osafune T. and Hase E. (1995)Behavior of mitochondria in synchronized cells of Chlamydomonas reinhardtii(Chlorophyta). J. Cell Sci. 108: 499-507.
Ehara, T., Osafune T. and Hase E. (1996)Association of nuclear and chloroplast DNA molecules in synchronized cells of Chlamydomonas reinhardtii. J. Electron Microsc., 45: 159-162
Kuroiwa, T. (1982)Mitochondrial nuclei, Int. Rev. Cytol., 75: 1-59.
Mihara S. and Hase E. (1971)Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture I. Plant Cell Physiol., 12: 225-236.
Nass M. M. K. and Nass S. (1962)Fibrous structures within the matrix of developing chick embryo mitochondria. Expl, Cell Res., 26: 424-437.
Osafune T. (1973)Three-dimensional structures of giant mitochondria, dictyosomes and“ concentric lamellar bodies” formed during the cell cycle of Euglena gracilis(Z)in synchronized culture. J. Electron Microsc., 22: 51-61.
Osafune T., Mihara S., Hase E. and OhkuroI. (1975)Formation and division of giant mito chondria during the cell cycle of Euglena gracilis Z in synchronous culture I. Some characteristics of changes in the morphology of mitochondria and oxygen-uptake activity of cells. Plant Cell Physiol., 16: 313-326.
Osafune T., Ehara T., Hase E.and Schiff J. A. (1993)Stage-dependent association of nuclear and chloroplast DNA molecules through bridges transiently farmed between the two organelles in synchronized cells of Euglena gracilis. J. Electron Microsc., 42: 197-201.
Pellegrini M. (1980)Three-dimensional reconstruction of organelles in Euglena gracilis Z. I. Qualitative and quantitative changes of chloroplasts and mitochondrial reticulum in synchronous photoautotrophic culture. J. Cell Sci., 43: 137-166.
Ryan R. D., Grant K., Chiang S. and Swift H. (1978)Isolation and characterization of mito chondrial DNA from Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. 75: 3268-3272
Tamiya H. (1966)Synchronous cultures of algae. Ann. Rev. Plant Physiol., 17: 1-26.
Tsukada S., Ehara T., Sumida S., Osafune T. and Hase E. (1986)Behaviour of proplastids and their nucleoids in dark-organotrophically grown cells of Euglena gracilis transferred to an inorganic medium. Br. Phycol. J., 21: 155-163.

抄録

ミトコンドリアは細胞周期において、どのように形態変化をしながら娘細胞に分配されるのだろうか。われわれは単細胞緑藻Chlamydomonas reinhardtiiの細胞周期でミトコンドリアの形態がダイナミックに絶え間無く変化する姿を生体観察によって捕らえた。そして、細胞の成長期には呼吸活性の低下を伴って、巨大ミトコンドリア(塊状形態)が2度出現する現象を見出し、それらの動態を細胞周期中に位置づけた。また、電子顕微鏡による2次元的な超微形態観察からそれらの現象を裏付けると共に、連続超薄切片を作成してコンピュータ・グラフィックス(CG)で細胞の再構築をおこない、3次元解析をすることでミトコンドリアの全体像を明らかにした。Chlamydomonasの細胞周期中で起こるミトコンドリアの様々な動態を通して、独自の遺伝情報と発現系をもちながら半自律的に自己増殖するミトコンドリアが、どのようにして次世代へ連続性をもって受け継がれいくのか、その基本的な問題について考察する。

責任著者(Corresponding author)

訂正情報

J-STAGEへの登録はこちら（無料）