Polyethylene glycol embedding for immunofluorescence microscopy of plant tissues.

Koichi Uehara

doi:10.5685/plmorphol.3.31

植物組織研究におけるポリエチレングリコール包埋切片法の実際

上原浩一

著者情報

ジャーナルフリー

1991 年 3 巻 1 号 p. 31-35

DOI https://doi.org/10.5685/plmorphol.3.31

詳細

抄録

Polyethylene glycol embedding is an useful method for immunofluorescence microscopy of plant tissues. The dehydration, infiltration and embedding of materials into the polyethylene glycol is easier than the procedures in the usual paraffin method. And the sectioning is easier than cryosectioning. Serial sections of 5-10μm thickness are obtained with a rotary microtome for various tissues of higher plants. This method is useful for immunofluorescence microscopy of microtubules. Microtubule arrangements in elate formation of Equisetum spore are observed by this method.

責任著者(Corresponding author)

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