維管束植物組織研究における包埋法の重要性と系統解析への応用とその展開

抄録

An ordinary paraffin section method has been generally used for a variety of anatomical and histological studies of vascular plants. Recently, however, a semithin section method, which was originally developed for transmission electron microscopy, has been widely applied because of clearness of figures obtained. Materials embedded in Spurr's low-viscosity resin are cut at a thickness of 1-2μm, stained with toluidine blue 0, and observed under a light microscope. The procedures of the semithin section method I employed for a morphogenetic study of Selaginella rhizophores are reported here. Another section method using water-miscible methacrylate(Technovit 7100)is also useful for sectioning of relatively large materials. These methods were useful in recent anatomical studies of Selaginella rhizophores and leguminous seed coats.