抄録
We grew 1005 mutated scented japonica rice lines to the seventh steady genetic generation, and treated the seedlings with six true leaves with 300 mM NaCl for three days. Only the salt-tolerant line, SM61, survived. We obtained F1, F2 and F3 populations from the cross between SM61 and a salt-susceptible indica variety, TCS17. After culture with 200 mM NaCl for five days, SM61 and F1 (SM61×TCS17; TCS17×SM61) plants survived (R) while TCS17 plants did not (S). The R to S ratio in 513 F2 plants showed a good fit to the Mendelian 3 : 1 segregation ratio by a Chi-square test indicating that the salt-tolerance of SM61 was governed by a single dominant gene. The mutated salt-tolerance gene explained close to 100% of the total phenotypic variation, and was tightly linked to RM223 (marker) located on chromosome 8, which was different from the results of previous studies investigating the relationship of QTLs with salt tolerance. This is the first report of mapping tightly linked markers of a single dominant mutated salt-tolerance gene.
