抄録
Water pollution with pathogenic viruses has been frequently reported in recent years. Since conventionalwater and wastewater treament systems are not enough to inactivate or remove pathogenic viruses, a new technology for virus removal should be developed. In this study, virus-binding proteins (VBPs) were isolated from activated sludge culture with affinity chomatography. The ability of VBPs to capture intact particles of poliovirus I was confirmed with ELISA. SDS-PAGE analysis revealed that VBPs included a number of polypeptidesthat have molecular weight between 94.0 and 14.4k Da. The evaluation of surface charges of VBPs with ion exchange chromatography found that a majority of VBP molecules had a net negative charge under the condition of the affinity chromatography. On the other hand, the viral peptide, which was used as an affinity ligand, was expected to have a net negative charge. As a result, the VBPs must be captured by the viral peptide with stronger binding forces than the electrostatic repulsive force. The affinity adsorption could explain the strong attractive interaction between VBPs and viral peptide. These VBPs could be useful as an innovative material for the virus removal.
