Abstract
We propose the spectroscopy-tomography of single cells to improve the early detection and treatment of cancer. This technology can obtain the three dimensional distribution of components at a high spatial resolution. In this paper, we mention the analysis result of the cross-sectional images of the microsphere which diameter is 10 μm. We confirmed that the distribution of the internal defect in the microsphere can be analyzed. And we mention the proposed variable phase-contrast spectrometry as the two dimensional high spatial resolution spectrometry. This proposed method is a phase-shift interferometry between the 0th order diffracted light and higher order diffracted light. We discuss the experimental result of the spectral characteristics using the proposed variable phase-contrast spectrometry.