Microneedles Based Cellular Thermometer for Living Cells: A Novel Approach

抄録

Cellular thermometry has a great demand in the clinical treatments and diagnosis of diseases. Microneedle type sensors gains more attention in contrast to conventional sensors due to its ability to penetrate the samples. As perforation takes only few seconds with microneedles, measurement and analysis could be quite simple. Besides that, the small dimensions of the sensor could be used for more precise measurements at single cell levels. In this work, microneedle based temperature sensor was developed to monitor the intercellular temperature. Microneedle was fabricated by simple electrochemical polishing of tungsten rod and temperature sensing electrodes was made u antimony (Sb) and sliver (Ag) by RF sputtering technique. The structural and morphological properties were analysed using XRD and FE-SEM. The microneedle tip size and angle was about 5 μm. The real time testing of the developed microneedle based temperature was employed to monitor the temperature of embryo of salmon fish and human lung cancer cells at controlled environment. The reported sensor here is totally reversible and results were reproducible after several routine testing.