抄録
Purpose : Alcohol dehydrogenase (ADH) is the principal enzyme responsible for ethanol oxidation. It is well established that ethanol preferences are important factors for evaluating the ethanol drinking capacity. However, their relationships are unknown. Therefore, the present study examined to clarify the relation between C57BL/6J and DBA/2J mice (7 wks old) in the ADH activity and the preference for ethanol. Methods and Materials : The mice were sacrificed by cervical dislocation and the livers were removed, weighed, and perfused with ice-cold 0.9% NaCl solution. 10% homogenates were prepared in ice-cold 0.1% Triton X-100 in 0.9% NaCl solution. The homogenate was centrifuged. The pellet was discarded, and the supernatant was spun. The resulting supernatant cytosolic fraction was used for the determination of ADH activity (38°C, pH9.0). The ADH activities were expressed as nM of NADH per min per mg protein. Results : The liver weight per body weight showed no significant difference. However, the ADH specific activities and total ADH activities of both strain mice were ca.1.33 times higher in female than in male. The ADH specific activities and total ADH activities of male and female C57BL/6J mice were ca.1.6 times higher than DBA/2J mice. These results suggest that the liver cytosolic ADH activities depend upon the level of the preference for ethanol and are influenced by sex-hormones. [Jpn J Physiol 54 Suppl:S232 (2004)]
