抄録
We have developed a quantitative filtration apparatus to evaluate erythrocyte deformability, using a precision metal filter (nickel mesh) which we developed. The filtration system consists of a test unit (a vertical glass tube equipped with a nickel mesh at the bottom end), a negative pressure supply unit, and a measurement control and data analysis unit. The vertical-tube is connected to an air tank or reservoir through electromagnetic valves inside the negative pressure supply unit. The test material is sucked into the filter through the action of decreasing negative pressure. The device automatically gives a liquid level (height)-time relation and a pressure-flow rate relation, similar to gravity-based filtration. Unlike the gravity-based system, the experimental performance of this system is very good and reproducible. Nowadays, the physiologic significance and clinical benefit of nickel mesh filtration is evident. Thus, we believe that our new, highly quantitative and convenient apparatus will contribute to the evaluation of erythrocyte deformability for biorheology and related fields of medical science. [Jpn J Physiol 54 Suppl:S92 (2004)]
