Chemical compounds in breath originate mainly from volatile metabolic products in health and diseases. Among them, hydrogen and methane are believed to represent the conditions of gastro-intestinal systems in the presence of bacterial flora. On the other hands, carbon monoxide and nitric oxide are well-known bioactive chemicals to play important roles in physiological and pathophysiological conditions. Because matrix analysis based on simultaneous measurements of exhaled chemicals with such low mass numbers may give us important information of physical conditions, we conducted the following experiment.Nine hundreds and forty two subjects aged from 20 to 88 years old volunteered for the present study. After tooth brushing and gargling, fasted subjects breathed purified artificial air via a mouthpiece in a sitting position for 12 min, during which exhaled air was collected via one-way valve into the Douglas bag. Minute ventilation volume and concentrations of carbon dioxide and oxygen in exhaled air were monitored by respiratory metabolic analyzer with the chemical sensors. Concentrations of hydrogen, methane, carbon monoxide and nitric oxide in the bag were analyzed by gas chromatography and chemiluninescence methods. We will present the summarized results of the relationships between minute expired volumes of these compounds and such physical conditions as age, gender, laboratory data, scores of health-related questionnaires, diseases and disorders by using the multivariate analysis. [J Physiol Sci. 2006;56 Suppl:S75]