抄録
The effects of D-allose, one of the rare sugars, against ischemia injury in rat retina were determined. Retinal ischemia was induced by intraocular pressure to 130mmHg and maintaining that level for 45min. Morphological analysis was performed to study the effects of D-allose on histological changes in the rat retina induced by ischemia. Glutamate release from retina was monitored by using a microdialysis biosensor. Vitreal PO2 was measured oxygen sensitive microelectrode during and after ischemia. Seven days after ischemia, significant reductions in both number of ganglion cells and thickness of the inner plexiform layer were detected. Pretreatment of D-allose significantly inhibited the ischemic injury of the inner retina. A large amount of glutamate was released during ischemia. After recirculation, glutamate levels were increased again and reached a maximum in 20min. This increase of released glutamate was clearly suppressed by the pretreatment of 200mg/kg D- allose. D-allose also attenuated the increase in vitreal PO2 values during repurfusion. These results suggest that D-allose may protect neurons by decreasing extra-cellular glutamate and attenuating oxidative stress in ischemic injury. injury in rat brain. [J Physiol Sci. 2006;56 Suppl:S210]
