1987 年 48 巻 2 号 p. 137-140
A dialysis culture chamber was constructed and used to determine cellular N/C and P/C ratios and in situ growth rate of individual phytoplankton species. The chamber was made of acrylic tubing and membrane filters of 47 mm in diameter for the diffusion boundary. A much higher permeation rate of dissolved ion into the chamber was obtained when the Nuclepore filters (0.2 and 3.0μm porosity) were used as the boundary, compared to the case when the cellophane membmane was used. Six species of planktonic algae were separatey incubated in the chamber equipped with 1.0μm porosity Nuclepore filters and suspended in a eutrophic pond for four days. The species specific growth rate and the cellular N/C and P/C ratios of these algal populations were obtained. The use of the dialysis culture chamber was thought to be effective in studying chemical composition of the species population in the fields. A few comments on the field use of dialysis culture chambers were made.
