シンポジウムIII 慢性骨髄性白血病治療の問題点
2. 細胞回転の立場から
1978 年 19 巻 4 号 p. 379-385
詳細
1978 年 19 巻 4 号 p. 379-385
Cellular kinetics in chronic myelocytic leukemia (CML) was studied by flow microfluorometry (F. M. F.) which showed DNA histogram and TLC separation of dansyl polyamines (Putresine, Spermidine and Spermine).
Normal blood, which consisted of mature cells alone, had monopeak of diploid (2N), and normal bone marrow, which consisted dividing compartment (myeloblasts, promyelocytes, myelocytes and immature erythroblasts), had two peaks of diploid and tetraploid (4N). AML, ALL and CLL had the same DNA histogram as normal blood and bone marrow, because leukemic cells in blood were long resting cells (nondividing compartment).
CML blood with splenomegaly had two peaks as bone marrow. But CML blood without splenomegaly or post-splenectomy had diploid peak alone as normal blood.
Leukemoid reaction and polycythemia vera blood, which contained immature cells as CML blood, had also diploid peak alone, but myelofibrosis blood had two peaks.
In some AML polyamine contents level were very high, but in CML and others not so high. In general, polyamine contents were higher in the bone marrow than in the blood (PB<BM), but in some cases with CML they were higher in the blood than in the bone marrow (PB>BM) at chronic phase and PB<BM at remission and PB>BM again at blastic crisis.
As a conclusion, it was guessed that dividing compartment of immature cells in CML blood came out of spleen and/or liver and nondividing compartment of immature cells and mature cells came out of spleen and/or liver and bone marrow.
