抄録
The functions of platelets collected by the Haemonetics blood processor Model 30 in 20 healthy volunteers were investigated. Aggregability and release ability of these platelets were compared with platelets from the same donors before plateletpheresis. ADP-, epinephrine- or collagen-induced platelet aggregation was markedly decreased in platelets isolated by Haemonetics Model 30. It was, however, less reduced than that of platelets obtained from Japan Red Cross Blood Center. ATP release induced by collagen was also decreased when examined with Chrono-log lumiaggregometer. During plateletpheresis, significant amount of β-thromboglobulin and possibly ATP were released from platelets indicating that the activation of platelets occured. Haemonetics-isolated platelets were transfused into 5 year-old boy, in whom platelet count was only 1.0×104/μl due to aplastic anemia. Following transfusion platelet count was increased to 15×104/μl resulting in shortening of Ivy bleeding time from over 15 min. to 10 min. Aggregation of platelets obtained from the patient after transfusion was also considerably improved indicating that defects of Haemonetics-isolated platelet functions appeared reversible. Our study showed that Haemonetics-isolated platelets were hemostatically effective although they were defective in aggregation and release activity in vitro.
